期刊
ACS APPLIED MATERIALS & INTERFACES
卷 9, 期 14, 页码 12264-12271出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.7b00365
关键词
mesenchymal stem cell; collagen; hyaluronic acid; multilayer nanofilm; openness of film; mutlifunctional nanofilm
资金
- Basic Science Research Program through the National Research Foundation of Korea - MEST [2015R1D1A1A01059702]
- Midcareer Research Program through the National Research Foundation of Korea - MEST [NRF-2015R1A2A2A04006172]
- Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) - Ministry of Health & Welfare, Republic of Korea [HI14C-3266, HILSC-1653]
- Bio & Medical Technology Development Program of the National Research Foundation of Korea (NRF) - Korean Government [2012M3A9C6050104, 2016M3A9C6917405]
- Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) - Korea Ministry of Health Welfare [HI16C1010]
- National Research Foundation of Korea [2015R1D1A1A01059702] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Cell encapsulation has been reported to convey cytoprotective effects and to better maintain cell survival. In contrast to other studies, our report shows that the deposition of two major biomacromolecules, collagen type I (Col) and hyaluronic acid (HA), on mesenchymal stem cells (MSCs) does not entirely block the cell plasma membrane surface. Instead, a considerable amount of the surface remained uncovered or only slightly covered, as confirmed by TEM observation and by FACS analysis based on quantitative surface labeling. Despite this structure showing openness and flexibility, the multilayer Col/HA films significantly increased cell survival in the attachment deprived culture condition. In terms of stem cell characteristics, the MSCs still showed functional cell activity after film deposition, as evidenced by their colony-forming activity and in vitro osteogenic differentiation. The Col/HA multilayer films could provide a cytoprotective effect and induce osteogenic differentiation without deteriorating effect or inhibition of cellular attachment, showing that this technique can be a valuable tool for modulating stem cell activities.
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