期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 409, 期 9, 页码 2393-2400出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-017-0185-1
关键词
Cross-linking; Enrichment; Protein conformation; Protein-protein interactions; Solid phase extraction; Strong cation exchange
资金
- DFG [Si 867/15-2]
Chemical cross-linking/mass spectrometry (MS) is gradually developing into a routine method to investigate protein conformation and to decipher protein interaction networks. To increase identification rates of the frequently low abundant cross-linked products in LC/MS/MS experiments, fast and reliable sample preparation protocols are indispensable. We present simplified solid phase extraction methods using C18/SCX StageTips and mixed-mode OASIS MCX cartridges for a single-step enrichment of cross-linked products prior to LC/MS/MS analysis. Our improved protocols result in 3.5 to 4.6 times higher numbers of cross-link identifications for the model protein bovine serum albumin compared to nonprocessed samples.
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