期刊
ACS NANO
卷 11, 期 3, 页码 2764-2772出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.6b07806
关键词
nanoparticles; nanobio interactions; particokinetics; endothelial cells; VE-cadherin mechanics; biophysics
类别
资金
- Ministry of Education [MOE2013-T2-2-093]
The integrity of the vasculature system is intrinsically sensitive to a short list of biophysical cues spanning from nano to micro scales. We have earlier found that certain nanomaterials could induce endothelial leakiness (nano particle induced endothelial leakiness, nanoEL). In this study, we report that the density of the nanomaterial, a basic intrinsic material property not implicated in many nanoparticle-mediated biological effects, predominantly dictates the nanoEL effect. We demonstrated that the impinging force exerted by a library of increasing effective densities but consistently sized silica nanoparticles (SiNPs) could directly increase endothelial permeability. The crossover effective particle density that induced nanoEL was determined to be between 1.57 g/cm(3) to 1.72 g/cm(3). It was also found that a cumulative gravitational-mediated force of around 1.8 nN/mu m along the boundaries of the vascular endothelial cadherin (VE-cad) adherens junctions appeared to be a critical threshold force required to perturb endothelial cell cell adhesion. The net result is the snapping of the mechanically pretensed VE-cad (Nanosnap), leading to the formation of micron-sized gaps that would dramatically increase endothelial leakiness.
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