期刊
BLOOD
卷 129, 期 13, 页码 1840-1854出版社
AMER SOC HEMATOLOGY
DOI: 10.1182/blood-2016-07-729400
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资金
- Canadian Institutes of Health Research (MOP) [97918, 119540]
- Heart and Stroke Foundation of Canada (Ontario)
- Canadian Foundation for Innovation
- National Natural Science Foundation of China grant [31371163]
- Fundamental Research Funds for the Shenzhen of China [GJHZ201404221516216]
- Canadian Blood Services Postdoctoral Fellowship
- State Scholarship Fund from China Scholarship Council
- Ontario Trillium Scholarship, Canada
- Canadian Blood Services and Meredith & Malcolm Silver Scholarship in Cardiovascular Studies
- Heart and Stroke/Richard Lewar Centre of Excellence Studentship award
- Meredith & Malcolm Silver Scholarship in Cardiovascular Studies
- National Institutes of Health, National Institute of Allergy and Infectious Diseases [AI-044902]
Integrins are a large family of heterodimeric transmembrane receptors differentially expressed on almost all metazoan cells. Integrin beta subunits contain a highly conserved plexin-semaphorin-integrin (PSI) domain. The CXXC motif, the active site of the protein-disulfide-isomerase (PDI) family, is expressed twice in this domain of all integrins across species. However, the role of the PSI domain in integrins and whether it contains thiol-isomerase activity have not been explored. Here, recombinant PSI domains of murine beta 3, and human b1andb2 integrins were generated and their PDI-like activitywas demonstrated by refolding of reduced/denatured RNase. We identified that both CXXC motifs of beta 3 integrin PSI domain are required to maintain its optimal PDI-like activity. Cysteine substitutions (C13A and C26A) of the CXXC motifs also significantly decreased the PDI-like activity of full-length human recombinant beta 3 subunit. We further developed mouse anti-mouse beta 3 PSI domain monoclonal antibodies (mAbs) that cross-react with human and other species. These mAbs inhibited alpha IIb beta 3 PDI-like activity and its fibrinogen binding. Using single-molecular Biomembrane-Force-Probe assays, we demonstrated that inhibition of alpha IIb beta 3 endogenous PDI-like activity reduced alpha IIb beta 3-fibrinogen interaction, and these anti-PSI mAbs inhibited fibrinogen binding via different levels of both PDI-like activity-dependent and -independent mechanisms. Importantly, these mAbs inhibited murine/ human platelet aggregation in vitro and ex vivo, and murine thrombus formation in vivo, without significantly affecting bleeding time or platelet count. Thus, the PSI domain is a potential regulator of integrin activation and a novel target for antithrombotic therapies. These findings may have broad implications for all integrin functions, and cell-cell and cell-matrix interactions.
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