期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 56, 期 17, 页码 4802-4805出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201702415
关键词
cancer biomarkers; cancer pathway; single-cell imaging; two-stage disassembly
资金
- National Natural Science Foundation of China [21421004, 21327807, 21605048]
- Programme of Introducing Talents of Discipline to Universities [B16017]
- Program for Professor of Special Appointment (Eastern Scholar) at Shanghai Institutions of Higher Learning [YJ0130504]
- Program of Shanghai Subject Chief Scientist [15XD1501200]
- China Postdoctoral Science Foundation [2016T90339]
- State Key Laboratory of Analytical Chemistry for Life Science Open Foundation [SKLACLS1512]
The monitoring of cancer biomarkers is crucial to the early detection of cancer. However, a limiting factor in biomarker analysis is the ability to obtain the multilayered information of various biomarker molecules located at different parts of cells from the plasma membrane to the cytoplasm. A two-stage dissociation nanoparticle system based on multi-functionalized polydopamine-coated gold nanoparticles (Au@PDA NPs) is reported, which allows for the two-stage imaging of cancer biomarkers in single cells. We demonstrate the feasibility of this strategy on sialic acids (SAs), p53 protein, and microRNA-21 (miRNA-21) in MCF-7 breast cancer cells by two custom-built probes. Furthermore, the multicolor fluorescence information extracted is used for the monitoring of biomarker expression changes under different drug combinations, which allows us to investigate the complex interactions between various cancer biomarkers and to describe the cancer biomarker-synergic networks in single cells.
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