期刊
ACS APPLIED MATERIALS & INTERFACES
卷 9, 期 12, 页码 10554-10566出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.7b01886
关键词
sugar; osmolyte; nanoparticle; chaperone; amyloid fibril; Huntington's disease
资金
- Deparnnent of Biotechnology (DBT), Government of India [SR/NM/NS-1143/2016]
Sugar-based osmolyte molecules are known to stabilize proteins under stress, but usually they have poor chaperone performance in inhibiting protein aggregation. Here, we show that the nanoparticle form of sugars molecule can enhance their chaperone performance typically by 10(2)-10(5) times, compared to molecular sugar. Sugar-based plate-like nanopartides of 20-40 nm hydrodynamic size have been synthesized by simple heating of acidic aqueous solution of glucose/sucrose/maltose/trehalose. These nanoparticles have excitation-dependent green/yellow/orange emission and surface chemistry identical to the respective sugar molecule. Fibrillation of lysozyme/insulin/amyloid beta in extracellular space, aggregation of mutant huntingtin protein inside model neuronal cell, and cytotoxic effect of fibrils are investigated in the presence of these sugar nanoparticles. We found that sugar nanoparticles are 10(2)-10(5) times efficient than respective sugar molecules in inhibiting protein fibrillation and preventing cytotoxicity arising of fibrils. We propose that better performance of the nanoparticle form is linked to its stronger binding with fibril structure and enhanced cell uptake. This result suggests that nanoparticle form of osmolyte can be an attractive option in prevention and curing of protein aggregation-derived diseases.
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