期刊
BIOSENSORS & BIOELECTRONICS
卷 91, 期 -, 页码 560-565出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2017.01.012
关键词
Genetically-modified organisms; DNA; Phosphorescent resonance energy transfer; Room-temperature phosphorescence quantum dots
类别
资金
- Fund for Construction Program of Chemical Advantage and Key Discipline of Shanxi Province of China [912019]
A QDs-DNA nano-probe was made by combining Mn-doped ZnS room-temperature phosphorescence (RTP) quantum dots (QDs) and DNA. Then an RTP sensor for quantitative detection of genetically-modified mark sequence cauliflower mosaic virus 35S promoter (Ca MV 35S) DNA was built on basis of phosphorescent resonance energy transfer (PRET). The underlying principles were that a QDs-DNA water-soluble nano-probe was built by connecting single-strand DNA to the surfaces of QDs via a ligand exchange method. This probe had good RTP performance and could well identify Ca MV 35 S. Thereby, the simple, rapid and efficient detection of genetically-modified organisms was realized. With the increase of target DNA sequence, the phosphorescent intensity of QDs was gradually reduced due to the energy transfer between QDs and the organic quencher BHQ(2). This sensor had a detection limit of 4.03 nM and a detection range of 12-300 nM. Moreover, this sensor had high selectivity. This sensor could effectively detect the target DNA compared with mismatched and random sequences. Thus, this method is very promising for biological analysis.
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