期刊
ACS APPLIED MATERIALS & INTERFACES
卷 9, 期 15, 页码 12959-12966出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.6b15212
关键词
nanoporous gold; electrochemical coarsening; nucleic acid based biosensors; high-throughput material screening; biofouling; multiple electrode array
资金
- University of California Lab Fees Research Program [12-LR-237197]
- University of California-Davis Research Investments in the Sciences Engineering
- National Science Foundation [CBET-1512745, CBETDMR-1454426]
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [1454426] Funding Source: National Science Foundation
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [1512745] Funding Source: National Science Foundation
Nanoporous gold (np-Au) electrode coatings significantly enhance the performance of electrochemical nucleic acid biosensors because of their three-dimensional nanoscale network, high electrical conductivity, facile surface functionalization, and biocompatibility. Contrary to planar electrodes, the np-Au electrodes also exhibit sensitive detection in the presence of common biofouling media due to their porous structure. However, the pore size of the nanomatfix plays a critical role in dictating the extent of biomolecular capture and transport. Small pores perform better in the case of target detection in complex samples by filtering out the large nonspecific proteins. On the other hand, larger pores increase the accessibility of target nucleic acids in the nanoporous structure, enhancing the detection limits of the sensor at the expense of more interference from biofouling molecules. Here, we report a microfabricated np-Au multiple electrode array that displays a range of electrode morphologies on the same chip for identifying feature sizes that reduce the nonspecific adsorption of proteins but facilitate the permeation of target DNA molecules into the pores. We demonstrate the utility of the electrode morphology library in studying DNA functionalization and target detection in complex biological media with a special emphasis on revealing ranges of.electrode morphologies that mutually enhance the limit of detection and biofouling resilience. We expect this technique to assist in the development of high-performance biosensors for point-of-care diagnostics and facilitate studies on the electrode structure property relationships in potential applications ranging from neural electrodes to catalysts.
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