期刊
DOMESTIC ANIMAL ENDOCRINOLOGY
卷 59, 期 -, 页码 53-57出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.domaniend.2016.11.003
关键词
Steroid; Cortisol; HPA axis; Stress; Canine; Keratin
资金
- Edward W. and Stella C. Van Houten Memorial Fund through student-faculty collaborative scholarship
- Office of the Dean at Rollins College
Cortisol, a glucocorticoid secreted in response to stress, is used to assess adrenal function and mental health in clinical settings. Current methods assess cortisol sources that reflect short-term secretion that can vary with current stress state. Here, we present a novel method for the extraction and quantification of cortisol from the dog nail using solid phase extraction coupled to enzyme-linked immunosorbent assay. Validation experiments demonstrated accuracy (r = 0.836, P < 0.001) precision (15.1% coefficients of variation), and repeatability (14.4% coefficients of variation) with this method. Furthermore, nail cortisol concentrations were positively correlated to an established hair cortisol method (r = 0.736, P < 0.001). Nail cortisol concentrations did not differ with dog sex, breed, age, or weights; however, sample size limitations may preclude statistical significance. Nail cortisol may provide information on cortisol secretion integrated over the time corresponding to nail growth and may be useful as a tool for diagnosing stress and adrenal disorders in dogs. (C) 2016 Elsevier Inc. All rights reserved.
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