期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 152, 期 -, 页码 169-175出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2017.01.028
关键词
Magnetofection; Gene delivery; Superparamagnetism; Calf thymus DNA; Chitosan
资金
- Materials and Energy Research Center
Superparamagnetic iron oxide nanoparticles (MNPs) were prepared and coated with chitosan (CS). The chitosan-magnetic iron oxide nanoparticles (CS-MNPs) were characterized using Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), vibrating sample magnetometry (VSM), and the morphology of the particles was studied by transmission electron microscopy (TEM). Our findings show that the magnetic particles were monodisperse (10 nm mean diameter) and exhibited superparamagnetic behavior. The interaction between the particles and calf-thymus DNA (DNA) in physiological buffer was studied with UV-vis, fluorescence and circular dichroism spectroscopy and zeta potential. Spectroscopic studies were indicated DNA conformational changes in the presence of CS-MNPs. Binding and thermodynamic parameters at different temperatures were calculated using the Stern-Volmer, Hill, Scatchard and Van't Hoff equations. The binding process was spontaneous and interactions were electrostatic with the appropriate binding constant (K-b =4.52 x 10(3) M-1, 3.69 x 10(3) M-1 and 3.02 x 10(3) M-1 at 300 K, 310 K and 320 K, respectively). Zeta potential measurements of DNA continually increased with the addition of CS-MNPs, supporting our thermodynamic findings. Moreover, CS-MNPs were able to quench the fluorescence of DNA-intercalated ethidium bromide (DNA-EB) by a static quenching mechanism. Cytotoxicity studies show that the DNA-CS-MNP system is biocompatible with a human foreskin fibroblast cell line, HFFF2. Collectively, these results suggest that surface cationic magnetic chitosan-iron oxide nanoparticles can potentially enhance magnetofection efficiency. (C) 2017 Elsevier B.V. All rights reserved.
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