期刊
GENOME MEDICINE
卷 9, 期 -, 页码 -出版社
BIOMED CENTRAL LTD
DOI: 10.1186/s13073-017-0467-4
关键词
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资金
- Australian National Health and Medical Research Council [1028641, 1126399]
- University of Queensland, Australian Infectious Disease Research Centre [1028643]
- European Research Council grant ThSWITCH [260507]
- Lister Institute for Preventative Medicine
- National Health and Medical Research Council of Australia [1126399] Funding Source: NHMRC
- European Research Council (ERC) [260507] Funding Source: European Research Council (ERC)
RNA sequencing (RNA-seq) is a genomic approach for the detection and quantitative analysis of messenger RNA molecules in a biological sample and is useful for studying cellular responses. RNA-seq has fueled much discovery and innovation in medicine over recent years. For practical reasons, the technique is usually conducted on samples comprising thousands to millions of cells. However, this has hindered direct assessment of the fundamental unit of biology-the cell. Since the first single-cell RNA-sequencing (scRNA-seq) study was published in 2009, many more have been conducted, mostly by specialist laboratories with unique skills in wet-lab single-cell genomics, bioinformatics, and computation. However, with the increasing commercial availability of scRNA-seq platforms, and the rapid ongoing maturation of bioinformatics approaches, a point has been reached where any biomedical researcher or clinician can use scRNA-seq to make exciting discoveries. In this review, we present a practical guide to help researchers design their first scRNA-seq studies, including introductory information on experimental hardware, protocol choice, quality control, data analysis and biological interpretation.
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