期刊
G3-GENES GENOMES GENETICS
卷 7, 期 3, 页码 923-933出版社
GENETICS SOCIETY AMERICA
DOI: 10.1534/g3.116.038539
关键词
null allele; Orai; SOCE; tyrosine hydroxylase; hypoderm
资金
- Department of Science and Technology
- NCBS, Tata Institute of Fundamental Research
- Council of Scientific and Industrial Research
Molecular components of store-operated calcium entry have been identified in the recent past and consist of the endoplasmic reticulum (ER) membrane-resident calcium sensor STIM and the plasma membrane-localized calcium channel Orai. The physiological function of STIM and Orai is best defined in vertebrate immune cells. However, genetic studies with RNAi strains in Drosophila suggest a role in neuronal development and function. We generated a CRISPR-Cas-mediated deletion for the gene encoding STIM in Drosophila (dSTIM), which we demonstrate is larval lethal. To study STIM function in neurons, we merged the CRISPR-Cas9 method with the UAS-GAL4 system to generate either tissue- or cell type-specific inducible STIM knockouts (KOs). Our data identify an essential role for STIM in larval dopaminergic cells. The molecular basis for this cell-specific requirement needs further investigation.
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