期刊
G3-GENES GENOMES GENETICS
卷 7, 期 11, 页码 3831-3836出版社
GENETICS SOCIETY AMERICA
DOI: 10.1534/g3.117.300107
关键词
genome assembly; fungal genomics; nanopore sequencing; pathogen genomics
资金
- National Institutes of Health [R01-HL129239, R01-HG006677, T32-AI007417]
Here we describe the sequencing and assembly of the pathogenic fungus Lomentospora prolificans using a combination of short, highly accurate Illumina reads and additional coverage in very long Oxford Nanopore reads. The resulting assembly is highly contiguous, containing a total of 37,627,092 bp with over 98% of the sequence in just 26 scaffolds. Annotation identified 8896 protein-coding genes. Pulsed-field gel analysis suggests that this organism contains at least 7 and possibly 11 chromosomes, the two longest of which have sizes corresponding closely to the sizes of the longest scaffolds, at 6.6 and 5.7 Mb.
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