4.3 Article

First Draft Genome Sequence of the Pathogenic Fungus Lomentospora prolificans (Formerly Scedosporium prolificans)

期刊

G3-GENES GENOMES GENETICS
卷 7, 期 11, 页码 3831-3836

出版社

GENETICS SOCIETY AMERICA
DOI: 10.1534/g3.117.300107

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genome assembly; fungal genomics; nanopore sequencing; pathogen genomics

资金

  1. National Institutes of Health [R01-HL129239, R01-HG006677, T32-AI007417]

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Here we describe the sequencing and assembly of the pathogenic fungus Lomentospora prolificans using a combination of short, highly accurate Illumina reads and additional coverage in very long Oxford Nanopore reads. The resulting assembly is highly contiguous, containing a total of 37,627,092 bp with over 98% of the sequence in just 26 scaffolds. Annotation identified 8896 protein-coding genes. Pulsed-field gel analysis suggests that this organism contains at least 7 and possibly 11 chromosomes, the two longest of which have sizes corresponding closely to the sizes of the longest scaffolds, at 6.6 and 5.7 Mb.

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