期刊
PROTEOMES
卷 5, 期 1, 页码 -出版社
MDPI
DOI: 10.3390/proteomes5010004
关键词
isoelectric focusing; proteomics; electrophoresis; two-dimensional gel electrophoresis; isoelectric trapping; capillary isoelectric focusing
资金
- Department of Chemistry, College of Liberal Arts and Sciences, University of Illinois at Chicago
The separation of ampholytic components according to isoelectric point has played an important role in isolating, reducing complexity and improving peptide and protein detection. This brief review outlines the basics of isoelectric focusing, including a summary of the historical achievements and considerations in experimental design. Derivative methodologies of isoelectric focusing are also discussed including common detection methods used. Applications in a variety of fields using isoelectric point based separations are provided as well as an outlook on the field for future studies.
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