期刊
ANALYTICA CHIMICA ACTA
卷 963, 期 -, 页码 164-174出版社
ELSEVIER
DOI: 10.1016/j.aca.2017.02.005
关键词
Chiral separations; Superficially porous particles; High-efficiency chiral stationary phase; Ultra-high performance liquid; chromatography (UHPLC); Sub/ supercritical fluid chromatography (SFC); Kinetic plots
资金
- Welch Foundation [Y0026]
Two new anion-exchange columns were prepared by bonding tert-butyl carbamoylated quinine to 2.7 mu m superficially porous particle (SPP) silica to create chiral stationary phases for high-efficiency and ultrafast chromatography. Performance and retention parameters of these new columns are compared with an analogous 5 mu m fully porous particle (FPP) based Chiralpak QNAX column and a 3-4 fold increase in efficiency was observed. Ultrafast separations ranging from 12 s down to sub-second are shown using 2.7 mu m SPPs bonded via hydrosilation to the selector. Potential benefits of 2.7 mu m SPP based columns for increased LC-MS compatibility were investigated. A van Deemter plot comparison showed 2.7 mu m SPP based columns provided a lower reduced plate height and a higher optimal linear velocity compared to the 5 mm FPP based column. With geometry-independent kinetic plots, 2.7 mu m SPP and 5 mu m FPP based columns were assessed for their kinetic performance and the maximal number of plates each column can generate in a given analysis time. The 2.7 mu m SPP based column showed remarkable performance improvements in speed and efficiency as indicated by the kinetic plots. (C) 2017 Elsevier B.V. All rights reserved.
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