Immobilization on Metal-Organic Framework Engenders High Sensitivity for Enzymatic Electrochemical Detection

The protection effect of metal organic framework (MOF) provides high stability for immobilized enzyme. The small cavities of MOFs, however, usually result in decreased apparent substrate affinity and enzymatic activity of immobilized enzyme, compared to native enzyme. We synthesized zeolitic imidazolate framework-8 (ZIF-8) with a combination of mesoporous and micropoimis channels for cytochrome c (Cyt c) immobilization. Compared with native Cyt c, the immobilized Cyt c displayed increased apparerit substrate affinity (Michaelis constant Km reduced by similar to 50%), similar to 128% increased enzymatic activity, and 1.4-fold increased sensitivity in the enzymatic electrochemical detection of H2O2. The immobilized Cyt c-coated screen-printed electrode was applied for the fast detection of residual H2O2 in microliter food samples such as milk and beer, making it promising for the development of efficient biosensors.