期刊
BRITISH JOURNAL OF DERMATOLOGY
卷 176, 期 5, 页码 1259-1269出版社
WILEY
DOI: 10.1111/bjd.15087
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资金
- Dermatology Foundation
- Medical Research Council [G1000846]
- British Skin Foundation
- National Institutes of Health/National Institute of Arthritis and Musculoskeletal and Skin Diseases [P20AR044535]
- Medical Research Council [G1000846] Funding Source: researchfish
- MRC [G1000846] Funding Source: UKRI
BackgroundThe method of generating bioengineered skin constructs was pioneered several decades ago; nowadays these constructs are used regularly for the treatment of severe burns and nonhealing wounds. Commonly, these constructs are comprised of skin fibroblasts within a collagen scaffold, forming the skin dermis, and stratified keratinocytes overlying this, forming the skin epidermis. In the past decade there has been a surge of interest in bioengineered skins, with researchers seeking alternative cell sources, or scaffolds, from which constructs can be established, and for more biomimetic equivalents with skin appendages. ObjectivesTo evaluate whether human hair follicle dermal cells can act as an alternative cell source for engineering the dermal component of engineered skin constructs. MethodsWe established in vitro skin constructs by incorporating into the collagenous dermal compartment: (i) primary interfollicular dermal fibroblasts, (ii) hair follicle dermal papilla cells or (iii) hair follicle dermal sheath cells. In vivo skins were established by mixing dermal cells and keratinocytes in chambers on top of immunologically compromised mice. ResultsAll fibroblast subtypes were capable of supporting growth of overlying epithelial cells, both in vitro and in vivo. However, we found hair follicle dermal sheath cells to be superior to fibroblasts in their capacity to influence the establishment of a basal lamina. ConclusionsHuman hair follicle dermal cells can be readily interchanged with interfollicular fibroblasts and used as an alternative cell source for establishing the dermal component of engineered skin both in vitro and in vivo.
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