期刊
BIOMEDICINE & PHARMACOTHERAPY
卷 89, 期 -, 页码 957-965出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.biopha.2017.01.137
关键词
Glioblastoma; Progression; MiR-338-5p; EFEMP1
资金
- National Natural Science Foundation of China [81301051]
Objective: We aimed to investigate the effect of miR-338-5p on proliferation, migration and invasion of glioblastoma (GBM) cells by regulating EFEMP1. Methods: The expression of miR-338-5p and EFEMP1 was measured by qRT-PCR and western blot. Transfection was conducted to regulate the expression of miR-338-5p and EFEMP1 in U87 cell lines. Cell proliferation, apoptosis, migration and invasion were evaluated using CCK-8 assay, flow cytometry and Transwell assay respectively. Dual luciferase reporter assay was performed to verify whether miR-338-5p directly targeted EFEMP1. Results: MiR-338-5p was significantly down-regulated in human GBM tumor tissues and cells while EFEMP1 was strongly upregulated (P < 0.05). Upregulated miR-338-5p was able to suppress cell proliferation, migration, invasion, and promote cell apoptosis in GBM cells (P < 0.05). Dual luciferase reporter gene assay determined that miR-338-5p directly targeted EFEMP1 (P < 0.05). Conclusions: MiR-338-5p suppressed proliferation, migration and invasion of GBM cells through inhibiting EFEMP1. (c) 2017 Published by Elsevier Masson SAS.
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