期刊
CLINICAL SCIENCE
卷 131, 期 9, 页码 865-881出版社
PORTLAND PRESS LTD
DOI: 10.1042/CS20170083
关键词
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资金
- Czech Science Foundation [13-10267S, 14-36804G]
- Ministry of Education, Youth and Sports of the Czech Republic within the ERC CZ program [LL1204]
- Grant Agency of the Charles University in Prague [1214214]
- Institute of Physiology, Czech Academy of Sciences [67985823]
- Institute for Clinical and Experimental Medicine - IKEM [00023001]
- European Commission [CZ. 2.16/3.1.00/28025]
Mitochondria play an essential role in improved cardiac ischaemic tolerance conferred by adaptation to chronic hypoxia. In the present study, we analysed the effects of continuous normobaric hypoxia (CNH) on mitochondrial functions, including the sensitivity of the mitochondrial permeability transition pore (MPTP) to opening, and infarct size (IS) in hearts of spontaneously hypertensive rats (SHR) and the conplastic SHR-mtBN strain, characterized by the selective replacement of the mitochondrial genome of SHR with that of the more ischaemia-resistant brown Norway (BN) strain. Rats were adapted to CNH (10% O-2, 3 weeks) or kept at room air as normoxic controls. In the left ventricular mitochondria, respiration and cytochrome c oxidase (COX) activity were measured using an Oxygraph-2k and the sensitivity of MPTP opening was assessed spectrophotometrically as Ca2+ -induced swelling. Myocardial infarction was analysed in anaesthetized open-chest rats subjected to 20 min of coronary artery occlusion and 3 h of reperfusion. The IS reached 68 +/- 3.0% and 65 +/- 5% of the area at risk in normoxic SHR and SHR-mtBN strains, respectively. CNH significantly decreased myocardial infarction to 46 +/- 3% in SHR. In hypoxic SHR-mtBN strain, IS reached 33 +/- 2% and was significantly smaller compared with hypoxic SHR. Mitochondria isolated from hypoxic hearts of both strains had increased detergent-stimulated COX activity and were less sensitive to MPTP opening. The maximum swelling rate was significantly lower in hypoxic SHR-mtBN strain compared with hypoxic SHR, and positively correlated with myocardial infarction in all experimental groups. In conclusion, the mitochondrial genome of SHR modulates the IS-limiting effect of adaptation to CNH by affecting mitochondrial energetics and MPTP sensitivity to opening.
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