期刊
CHEMOSPHERE
卷 175, 期 -, 页码 228-238出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.chemosphere.2017.02.042
关键词
Autophagy; LC3; ATG4; Pelteobagrus fulvidraco; Zinc; Exposure pathways
资金
- National Natural Science Foundation of China [31422056]
Autophagy mediates the regulation of lipid metabolism. Moreover, our recent study indicated that waterborne and dietborne zinc (Zn) exposure differentially influenced lipid metabolism in a fish species of significance for aquaculture, yellow catfish Pelteobagrus fulvidraco, but further mechanism remained unknown. The hypothesis of the present study is that autophagy mediated the Zn-induced changes of lipid metabolism of yellow catfish subjected to different exposure pathways. To this end, we cloned key genes involved in autophagy in yellow catfish, explored their mRNA expressions in responses to different Zn exposure pathways. Full-length cDNA sequences of two LC3 subtypes and six ATG4 isoforms were isolated from yellow catfish. More ATG4 members were firstly identified in fish that might have arisen by teleost-specific whole genome duplication events. All of these members shared similar domain structure to their orthologous genes of vertebrates. Their mRNAs were widely expressed in various tissues, but at variable levels. Extra Zn addition in water or diets induced (P < 0.05) mRNA expression of ATG4Da, ATG4Db and LOB. Considering their important roles of these genes in lipid metabolism, ATG4Da, ATG4Db and LC3B may mediate the changes of Zn-induced hepatic lipid metabolism of yellow catfish under different Zn exposure pathways. For the first time, we characterized the full-length cDNA sequences of six ATG4 isoforms and two LC3 subtypes, determined their tissue expression profiles and transcriptional responses to different Zn exposure pathways, which would contribute to our understanding of the molecular basis of autophagy, and also provide new insights into physiological responses to different Zn exposure pathways. (C) 2017 Elsevier Ltd. All rights reserved.
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