4.8 Article

Mitigating the Hook Effect in Lateral Flow Sandwich Immunoassays Using Real-Time Reaction Kinetics

期刊

ANALYTICAL CHEMISTRY
卷 89, 期 9, 页码 5095-5100

出版社

AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.7b00638

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资金

  1. National Science Foundation [CBET-1343058]
  2. National Institutes of Health [R01EB021331]
  3. Directorate For Engineering
  4. Div Of Chem, Bioeng, Env, & Transp Sys [1343058] Funding Source: National Science Foundation

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The quantification of analyte, concentrations Using lateral flow assays is a low-cost and user-friendly alternative to traditional lab-based assays. However, sandwich-type immuno-assays are often limited by the high-dose hook effect, which causes falsely low results when analytes are present at very high concentrations. In this paper, We present a reaction kinetics,based technique that solves this problem, significantly increasing the dynamic range of these devices. With the use of a traditional sandwich lateral flow immunoassay, a portable imaging device, and a Mobile interface, we demonstrate the technique by quantifying C-reactive protein concentrations in human serum, over a large portion of the physiological range. The technique could be applied to any hook effect-limited sandwich lateral flow assay and has a high level of accuracy,even in the hook effect range.

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