期刊
ANALYTICAL CHEMISTRY
卷 89, 期 9, 页码 5115-5123出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.7b00688
关键词
-
We demonstrate a novel strategy using affinity extraction (AE) LC-MS to directly measure drug exposure and target engagement, two, critical pharmacological questions, with a 'single'assay. The assay measures total drug and target concentration :at the;i-site of therapeutic action, as well as the amount of target bound to drug. The case study presented applies the strategy to measure drug engagement of a membrane bound receptor (CD40) that is critical to immune regulation in colon biopsies collected from monkey dosed with an anti-CD40 antibody. Unlike other techniques that measure receptor occupancy, such as flow cytometry, this technique does hot rely on-viable cells allowing measurement of frozen samples in a remote setting from the clinic.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据