期刊
CELL REPORTS
卷 19, 期 13, 页码 2836-2852出版社
CELL PRESS
DOI: 10.1016/j.celrep.2017.06.014
关键词
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类别
资金
- European Research Council (ERC) [646604, 648235]
- Excellence Initiative of the German federal and state governments [EXC 294, GSC-4]
- Deutsche Forschungsgemeinschaft [PF 202/8-1, WA 1598/5-1]
- Sonderforschungsbereiche [746, 1140, 1190]
Mitochondria perform central functions in cellular bioenergetics, metabolism, and signaling, and their dysfunction has been linked to numerous diseases. The available studies cover only part of the mitochondrial proteome, and a separation of core mitochondrial proteins from associated fractions has not been achieved. We developed an integrative experimental approach to define the proteome of east mitochondria. We classified > 3,300 proteins of mitochondria and mitochondriaassociated fractions and defined 901 high-confidence mitochondrial proteins, expanding the set of mitochondrial proteins by 82. Our analysis includes protein abundance under fermentable and nonfermentable growth, submitochondrial localization, single-protein experiments, and subcellular classification of mitochondria-associated fractions. We identified mitochondrial interactors of respiratory chain supercomplexes, ATP synthase, AAA proteases, the mitochondrial contact site and cristae organizing system (MICOS), and the coenzyme Q biosynthesis cluster, as well as mitochondrial proteins with dual cellular localization. The integrative proteome provides a highconfidence source for the characterization of physiological and pathophysiological functions of mitochondria and their integration into the cellular environment.
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