期刊
CELL REPORTS
卷 18, 期 5, 页码 1200-1214出版社
CELL PRESS
DOI: 10.1016/j.celrep.2017.01.018
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资金
- Swiss National Science Foundation [310030B_156936]
- Human Frontier Science Program [RGP-0016]
- Novartis Research Foundation
- Swiss National Science Foundation (SNF) [310030B_156936] Funding Source: Swiss National Science Foundation (SNF)
Chromatin moves with subdiffusive and spatially constrained dynamics within the cell nucleus. Here, we use single-locus tracking by time-lapse fluorescence microscopy to uncover information regarding the forces that influence chromatin movement following the induction of a persistent DNA double-strand break (DSB). Using improved time-lapse imaging regimens, we monitor trajectories of tagged DNA loci at a high temporal resolution, which allows us to extract biophysical parameters through robust statistical analysis. Polymer modeling based on these parameters predicts chromatin domain expansion near a DSB and damage extrusion from the domain. Both phenomena are confirmed by live imaging in budding yeast. Calculation of the anomalous exponent of locus movement allows us to differentiate forces imposed on the nucleus through the actin cytoskeleton from those that arise from INO80 remodeler-dependent changes in nucleosome organization. Our analytical approach can be applied to high-density single-locus trajectories obtained in any cell type.
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