期刊
CELL REPORTS
卷 21, 期 13, 页码 3957-3969出版社
CELL PRESS
DOI: 10.1016/j.celrep.2017.11.111
关键词
-
类别
资金
- Ministry of Science and Technology of China [2016YFA0100400]
- National Natural Science Foundation of China [31721003, 31430056, 91319306, 31401266, 31322031, 31371288]
- Science and Technology Commission of Shanghai Municipality [15XD1503500, 14CG16]
- Shanghai Pujiang Talent Project [14PJ1402900]
- East China Normal University (ECNU)
Pre-implantation embryo development is an intricate and precisely regulated process orchestrated by maternally inherited proteins and newly synthesized proteins following zygotic genome activation. Although genomic and transcriptomic studies have enriched our understanding of the genetic programs underlying this process, the protein expression landscape remains unexplored. Using quantitative mass spectrometry, we identified nearly 5,000 proteins from 8,000 mouse embryos of each stage (zygote, 2-cell, 4-cell, 8-cell, morula, and blastocyst). We found that protein expression in zygotes, morulas, and blastocysts is distinct from 2- to 8-cell embryos. Analysis of protein phosphorylation identified critical kinases and signal transduction pathways. We highlight key factors and their important roles in embryo development. Combined analysis of transcriptomic and proteomic data reveals coordinated control of RNA degradation, transcription, and translation and identifies previously undefined exon-junction-derived peptides. Our study provides an invaluable resource for further mechanistic studies and suggests core factors regulating pre-implantation embryo development.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据