期刊
CELL REPORTS
卷 19, 期 8, 页码 1698-1709出版社
CELL PRESS
DOI: 10.1016/j.celrep.2017.05.008
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资金
- DFG grant [EH 192/5-1]
- TUD Elite University Support the Best program
- Recruitment Program of Global Experts in Shandong University
- International S&T Cooperation Program of China (ISTCP) [2015DFE32850]
- Internal Research Funding of the Witten/Herdecke University [UW/H IFF2014, IFF2017-17]
Adenoviruses (Ads) are large human-pathogenic double-stranded DNA (dsDNA) viruses presenting an enormous natural diversity associated with a broad variety of diseases. However, only a small fraction of adenoviruses has been explored in basic virology and biomedical research, highlighting the need to develop robust and adaptable methodologies and resources. We developed a method for high-throughput direct cloning and engineering of adenoviral genomes from different sources utilizing advanced linear-linear homologous recombination (LLHR) and linear-circular homologous recombination (LCHR). We describe 34 cloned adenoviral genomes originating from clinical samples, which were characterized by next-generation sequencing (NGS). We anticipate that this recombineering strategy and the engineered adenovirus library will provide an approach to study basic and clinical virology. High-throughput screening (HTS) of the reporter-tagged Ad library in a panel of cell lines including osteosarcoma disease-specific cell lines revealed alternative virus types with enhanced transduction and oncolysis efficiencies. This highlights the usefulness of this resource.
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