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Biotinylated Micro-RNA Pull Down Assay for Identifying miRNA Targets

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BIO-PROTOCOL
卷 7, 期 9, 页码 -

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BIO-PROTOCOL
DOI: 10.21769/BioProtoc.2253

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miRNA; Biotin-pulldown; miRNA-mRNA interaction; Transfection; Biotin labelling

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资金

  1. Department of Veterans Affairs, Career Development Award
  2. Department of Surgery, University of Maryland School of Medicine

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microRNA (miRNA) directly associates with its target transcripts (mRNA). This protocol describes a method for detection of direct interaction between miRNA and mRNA. The result of interaction helps screening the specific target mRNAs for a miRNA.

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