期刊
BIOSENSORS & BIOELECTRONICS
卷 93, 期 -, 页码 124-131出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2016.09.059
关键词
Immunosensor; Electrochemical impedance spectroscopy; Botulinum neurotoxin A; Impedimetric immunosensor; Lable free biosensors
类别
资金
- Research Council of Bu-Ali Sina University
- Center of Excellence in Development of Environmentally Friendly Methods for Chemical Synthesis (CEDEFMCS)
In this work, a novel nanocomposite film consisting of the Au nanoparticles/graphene-chitosan has been designed to construct an impedimetric immunosensor for a rapid and sensitive immunoassay of botulinum neurotoxin A (BoNT/A). BoNT/A antibody was immobilized on glassy carbon electrode modified with Au nanoparticles/graphene-chitosan for the signal amplification. The fabrication of immunosensor was extensively characterized by using transmission electron microscopy (TEM), scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray diffraction (XRD), Fourier transform infrared (FTIR), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). The impedance changes, due to the specific immuno-interactions at the immunosensor surface that efficiently restricted the electron transfer of redox probe Fe(CN)(6)(4-/3-) were utilized to detect BoNT/A. The measurements were highly targeted specific and linear with logarithmic BoNT/A concentrations in PBS, milk and human serum across a 0.27-268 pg mL(-1) range and associated with a detection limit of 0.11 pg mL(-1).
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