期刊
DISEASES OF AQUATIC ORGANISMS
卷 124, 期 3, 页码 223-232出版社
INTER-RESEARCH
DOI: 10.3354/dao03120
关键词
Bdellovibrio and like organisms; BALOs; Bacteriovorax; Vibrio parahaemolyticus; Acute hepatopancreatic necrosis disease; AHPND; Shrimp
资金
- CPF (Thailand) [A27134]
- Royal Golden Jubilee PhD Program, Thailand Research Fund [PHD/0213/2556]
Acute hepatopancreatic necrosis disease, a severe disease of shrimp, is caused by Vibrio parahaemolyticus (AHPND Vp), a halophilic bacterium harboring a plasmid that contains toxin genes homologous to Photorhabdus insect-related toxins. We obtained 9 isolates of Bdello vibrio and like organisms (BALOs) from water and sediment samples in Thailand. Using 16S rRNA sequencing, all of the organisms were identified as Bacteriovorax spp. and were able to attack all tested AHPND Vp isolates. In addition, their various susceptible hosts, including Gram-positive and Gram-negative bacteria, were observed. The optimal ratio for interaction between the Bacteriovorax isolate BV-A and AHPND Vp was determined to be 1: 10. The suitable conditions applied for co-culture between BV-A and AHPND Vp were 30 degrees C, 2% NaCl, and pH 7.6. The capability of BV-A to reduce numbers of AHPND Vp in vitro was observed in co-culture after incubation for 2 d and continued until the end of the incubation period. In vivo, BV-A was able to reduce mortality of shrimp post-larvae infected with AHPND Vp. In addition, BV-A significantly decreased the formation of biofilm by AHPND Vp. These findings provide evidence for using Bacteriovorax as a biocontrol of AHPND Vp in shrimp aquaculture.
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