期刊
BIOSENSORS & BIOELECTRONICS
卷 91, 期 -, 页码 431-435出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2016.12.058
关键词
Photonic crystal fiber; Immunosensor; Cancer biomarker detection
类别
资金
- National Natural Science Foundation of China [21475019, 31579099]
- Fundamental Research Funds for the Central Universities [2412015BJ010]
- Jilin Provincial Department of Education
- Jilin Provincial Key Laboratory of Micro-nano Functional Materials (Northeast Normal University)
We have developed a sensitive photonic crystal fiber (PCF)-based immunosensor for detection of alpha fetoprotein (AFP). The unique PCF possesses a morphology characterized by numerous pore structures and a large surface area-to-volume ratio, which can be used as an immune-reaction carrier to improve the sensitivity and reaction speed of AFP detection. The PCF-based immunosensor possesses a low limit of detection of 0.1 ng/mL, which is five times lower than that of the capillary-based sensor and 35 times lower than that of the traditional enzyme-linked immunosorbent assay. The wide linear dynamic range of 0.1-150 ng/mL makes the developed immunosensor suitable for clinical practice. The proposed method was successfully applied to AFP detection in a clinical serum sample with acceptable precision. It is indicated that the present PCF-based immunosensor could be used as an attractive analytical platform for sensitive and specific detection of cancer biomarkers.
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