期刊
BIOSENSORS & BIOELECTRONICS
卷 91, 期 -, 页码 687-691出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2017.01.038
关键词
Dual recognition; Aptamer; Molecularly imprinted; lincomycin; Energy transfer
类别
资金
- Fundamental Scientific Research Funds of the Chinese Academy of Tropical Agricultural Sciences [1630042015011, 1630042015009]
In this study, a biosensor with a dual recognition system comprising a molecularly imprinted polymer (MIP) and aptamers selective for lincomycin was fabricated. The MIP was synthesized by electropolymerization of carbon dots (C-dots)-tagged DNA aptamers combined with lincomycin and o-aminophenol on the goldnanoparticle-functionalized graphene oxide (Au-GO)-modified electrode. Electrogenerated chemiluminescence (ECL) resonance energy transfer was observed between Au-GO and C-dots. After the C-dots accepted the energy, they acted as a signal indicator and exhibited enhanced signal intensity in the presence of target lincomycin. When lincomycin was competitively bound to DNA aptamers and MIP, it blocked the transfer of energy, and a decreased ECL signal was observed. Hence, a dual recognition method for the detection of lincomycin is realized. Using this strategy, the sensor exhibited a linear ECL response to lincomycin at concentrations from 5.0 x 10(-12) mol/L to 1.0 x 10(-9) mol/L. The detection limit of this assay was found to be 1.6 x 10(-13) mol/L. This method was utilized to determine lincomycin residuals in meat samples with satisfactory results.
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