期刊
BIOSENSORS & BIOELECTRONICS
卷 91, 期 -, 页码 849-856出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2017.01.027
关键词
Peroxynitrite; Fluorescence; Sensor; Imaging
类别
资金
- Ministry of Science and Technology
- Ministry of Health and Welfare of the Republic of China [MOST 104-2113-M-009-012-MY2, MOST 103-2627-M-009-001, MOHW105-TDU-B-212-134005]
- National Chiao Tung University and Ministry of Education
Peroxynitrite (ONOO-) is an important species involved in many physiopathological processes. Progresses have been made in developing novel fluorescent probes to detect peroxynitrite with relatively high sensitivity and specificity. Herein, we report the synthesis, characterization and biological applications of a new boronate-based fluorescent probe, 4-MB. The studies showed that 4-MB exhibits a dual ratiometric and calorimetric response toward peroxynitrite due to ONOO--triggered oxidative reaction. A possible mechanism of the oxidation reaction was proposed and the reaction product was isolated and characterized using different spectroscopic methods. We have thoroughly demonstrated the utility of 4-MB for intracellular peroxynitrite imaging. Further, we showed that 4-MB can be potentially employed to visualize exogenous and endogenous peroxynitrite in RAW264.7 macrophages, EAhy926 cells, zebrafish and in live tissues from a high-fat diet-induced obese mouse model.
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