期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 56, 期 21, 页码 5812-5816出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201702114
关键词
antioxidants; fluorescent probes; gluthionine; ratiometric probes; redox homeostasis
资金
- Natural Science Foundation of China [NSFC. 21165019, 21662037]
- Youth Science Foundation [20160520003JH]
- Natural Science Foundation [20160101301JC]
- Creative Sci-Tech Innovation Research Program of Jilin Province [012015005]
- National Creative Research Initiative programs of the National Research Foundation of Korea (NRF) - Korean government (MSIP) [2012R1A3A2048814]
The ability to monitor and quantify glutathione (GSH) in live cells is essential in order to gain a detailed understanding of GSH-related pathological events. However, owing to their irreversible response mechanisms, most existing fluorescent GSH probes are not suitable for this purpose. We have developed a ratiometric fluorescent probe (QG-1) for quantitatively monitoring cellular GSH. The probe responds specifically and reversibility to GSH with an ideal dissociation constant (K-d) of 2.59 mM and a fast response time (t(1/2) = 5.82 s). We also demonstrate that QG-1 detection of GSH is feasible in a model protein system. QG-1 was found to have extremely low cytotoxicity and was applied to determine the GSH concentration in live HeLa cells (5.40 +/- 0.87 mM).
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