期刊
ACS APPLIED MATERIALS & INTERFACES
卷 9, 期 15, 页码 12967-12974出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.6b16434
关键词
coating polycation; single cells; bioprocessing; cellular uptake; temporary; bioprinting
资金
- Arthritis Research UK Tissue Engineering Centre [19429]
- EPSRC Centre for Innovative Manufacture in Medical Devices (MeDe Innovation) [EP/K029592]
- Newcastle University
- JGW Patterson Foundation Fellowship
- NC3Rs Training Fellowship
- Engineering and Physical Sciences Research Council [EP/K029592/1] Funding Source: researchfish
- National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs) [NC/P002412/1] Funding Source: researchfish
- EPSRC [EP/K029592/1] Funding Source: UKRI
Temporary single-cell coating is a useful tool for cell processing, allowing manipulation of cells to prevent cell attachment and agglomeration, before re-establishing normal cell function. In this work, a speckled coating method using a known polycation [poly(L-lysine), PLL] is described to induce cell surface electrostatic charges on three different cell types, namely, two bone cancer cell lines and fibroblast. The morphology of the PLL speckled coating on the cell surface, internalization and metabolization of the polymer, and prevention of cellular aggregations are reported. Polymer concentration was found to be the key parameter controlling both capsule morphology and cell health. This approach allows a temporary cell coating over the course of 1-2 h; with cells exhibiting phenotypically normal behavior after ingesting and metabolizing the polymer. The process offers a fast, and efficient alternative to aid single-cell manipulation for bioprocessing applications. Preliminary work on the application of PLL speckled cell: coating in enabling reliable bioprinting is also presented.
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