期刊
BIOCONJUGATE CHEMISTRY
卷 28, 期 4, 页码 1076-1083出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.bioconjchem.7b00005
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资金
- University of Utah
- Huntsman Cancer Institute
- Utah Science and Technology Research (USTAR) initiative
Oligonucleotide conjugates of small molecules are widely used in chemical biology and have found increasing interest in the context of DNA-encoded chemical libraries for drug discovery. Attachment of molecules to DNA bound to the solid support is an attractive small-molecule conjugation method that permits the use of organic solvents, rigorous reaction conditions, and simple workup. However, the conjugated structures must be resistant to the harsh DNA deprotection/cleavage conditions and the stabilities of building blocks under various deprotection conditions are mostly unexplored. In the present study, we analyzed the stability of 131 structurally diverse fragments that contain amides and amide-like elements during DNA deprotection protocols. Structural features susceptible to decomposition in DNA deprotection conditions were identified and a protocol that enabled the synthesis of DNA conjugates with labile fragments on solid support was identified.
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