期刊
ACS APPLIED MATERIALS & INTERFACES
卷 9, 期 19, 页码 16026-16034出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.7b03645
关键词
DNA nanostructures; microchannel; microarray; multiplex detection; metal ions
资金
- National Natural Science Foundation of China [21505045, 21675167, 21373260, 21305034, 31571014]
- Shanghai Pujiang Program [15PJ1401800, 16PJ1402700]
- China Postdoctoral Science Foundation [2015M581565]
- introduction of major R&D projects of Fujian province [201412005]
- Natural Science Foundation of Fujian Province of China [2015J01064]
- Academy of Finland [297580]
- Jane and Aatos Erkko Foundation [4704010]
The development of rapid and sensitive point-of-test devices for on-site monitoring of heavy-metal contamination has great scientific and technological importance. However, developing fast, inexpensive, and sensitive microarray sensors to achieve such a goal remains challenging. In this work, we present a DNA-nanostructured microarray (DNM) with a tubular three-dimensional sensing surface and an ordered nanotopography. This microarray enables enhanced molecular interaction toward the rapid and sensitive multiplex detection of heavy-metal ions. In our design, the use of DNA tetrahedral-structured probes engineers the sensing interface with spatially resolved and density-tunable sensing spots that improve the microconfined molecular recognition. A bubble-mediated shuttle reaction was used inside the DNM-functionalized microchannel to improve the target-capturing efficiency. Using this novel DNM biosensor, the sensitive and selective detection of multiple heavy-metal ions (i.e., Hg2+, Ag+, and Pb2+) was achieved within 5 min, the detection limit was down to 10, 10, and 20 nM for Hg2+, Ag+, and Pb2+, respectively. The feasibility of our DNM sensor was further demonstrated by probing heavy-metal ions in real water samples with a direct optical readout. Beyond metal ions, this unique DNM sensor can easily be extended to in vitro bioassays and clinical diagnostics.
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