期刊
AIP ADVANCES
卷 7, 期 9, 页码 -出版社
AMER INST PHYSICS
DOI: 10.1063/1.4986872
关键词
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资金
- Nakatani Foundation for advancement of measuring technologies in biomedical engineering
- Tateishi Science and Technology Foundation
- CREST, Japan Science and Technology Agency (JST)
- Japan Society for the Promotion of Science of Japan [26249051, 15K21164]
- Project for Promotion of Researches toward Creation of Humanophilic Science and Technology
- VLSI Design and Education Center (The University of Tokyo)
- Cadence Corporation
- Mentor Graphics Corporation
Although fluorescence microscopy is the gold standard tool for biomedical research and clinical applications, their use beyond well-established laboratory infrastructures remains limited. The present study investigated a novel on-chip cell analysis platform based on contact fluorescence microscopy and microfluidics. Combined use of a contact fluorescence imager based on complementary metal-oxide semiconductor technology and an ultra-thin glass bottom microfluidic chip enabled both to observe living cells with minimal image distortion and to ease controlling and handling of biological samples (e.g. cells and biological molecules) in the imaged area. A proof-of-concept experiment of on-chip detection of cellular response to endothelial growth factor demonstrated promising use for the recently developed on-chip cell analysis platform. Contact fluorescence microscopy has numerous desirable features including compatibility with plastic microfluidic chips and compatibility with the electrical control system, and thus will fulfill the requirements of a fully automated cell analysis system. (C) 2017 Author(s).
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