期刊
BIORESOURCE TECHNOLOGY
卷 232, 期 -, 页码 285-296出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2017.02.049
关键词
Heterotrophic nitrification; Aerobic denitrification; Hydroxylamine reductase; Enterobacter cloacae; qRT-PCR
资金
- AMAAS Nodal centre, Indian Council of Agricultural Research (ICAR), India
Heterotrophic bacterium, Enterobacter cloacae CF-S27 exhibited simultaneous nitrification and aerobic denitrification in presence of high concentration of hydroxylamine. With the initial nitrogen concentration of 100 mg L-1 h(-1), ammonium, nitrate and nitrite removal efficiencies were 81%, 99.9% and 92.8%, while the corresponding maximum removal rates reached as high as 11.6, 15.1 and 11.2 mg L-1 h(-1) respectively. Quantitative amplification by real time PCR and enzyme assay demonstrated that hydroxylamine reductase gene (hao) is actively involved in hetrotrophic nitrification and aerobic denitrification process of Enterobacter cloacae CF-S27. PCR primers were designed targeting amplification of hao gene from diversified environmental soil DNA. The strain Enterobacter cloacae CF-S27 significantly maintained the undetectable amount of dissolved nitrogen throughout 60 days of zero water exchange fish culture experiment in domestic wastewater. (C) 2017 Elsevier Ltd. All rights reserved.
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