期刊
ADVANCED HEALTHCARE MATERIALS
卷 6, 期 15, 页码 -出版社
WILEY
DOI: 10.1002/adhm.201700196
关键词
3D coculture; angiogenesis; lymphangiogenesis; microfluidics; tumor microenvironments
资金
- Basic Science Research Program through National Research Foundation of Korea (NRF) - Ministry of Science, ICT & Future Planning [NRF-2015R1A2A1A09005662, 2016R1A4A1010796]
- Interdisciplinary Research Initiatives Program from College of Engineering, Seoul National University
- Interdisciplinary Research Initiatives Program from College of Medicine, Seoul National University
- National Research Foundation of Korea [2015H1A2A1033808, 2015R1A2A1A09005662] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
The Tumor microenvironment (TME) is a complex, interacting system of the tumor and its surrounding environment. The TME has drawn more attention recently in attempts to overcome current drug resistance and the recurrence of cancer by understanding the cancer and its microenvironment systematically, beyond past reductionist approaches. However, a lack of experimental tools to dissect the intricate interactions has hampered in-depth research into the TME. Here, a biomimetic TME model using a microfluidic platform is presented, which enables the interaction between TME constituents to be studied in a comprehensive manner. Paracrine interactions of cocultured tumor cell lines (SK-OV-3, MKN-74, and SW620) with primary fibroblasts show marked morphological changes in the tumor cells, depending on the type of tumor cells, and, importantly, the composition of the extracellular matrix. Furthermore, this model allows direct observation of angiogenesis induced by the tumor-stroma interaction. Finally, reconstituting simultaneous angiogenesis and lymphangiogenesis induced by the tumorstromal interaction with TME mimicking extrinsic factors is enabled. It is believed that the in vitro biomimetic model and the experimental concepts described will help to shed light on the complex biology of the TME.
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