期刊
ADVANCED HEALTHCARE MATERIALS
卷 6, 期 19, 页码 -出版社
WILEY
DOI: 10.1002/adhm.201700344
关键词
bFGF; deep second-degree scalds; hydrogel cores; liposomes; silk fibroin
资金
- National Natural Science Foundation of China [81603036, 81571392]
- Zhejiang provincial program for the cultivation of high-level innovative health talents
- 151 talent project of Zhejiang province
- 551 talent project of Wenzhou
- key support of high-level talent innovation and technology project of Wenzhou
- Zhejiang Provincial Foundation for Health Department [2015ZDA023, 2016KYA136]
- Zhejiang Provincial Natural Science Foundation [LY14H280002]
- Wenzhou Bureau of Science and Technology [Y20160086]
- Wenzhou Medical University [QTJ15020]
How to maintain the stability of basic fibroblast growth factor (bFGF) in wounds with massive wound fluids is important to accelerate wound healing. Here, a novel liposome with hydrogel core of silk fibroin (SF-LIP) is successfully developed by the common liposomal template, followed by gelation of liquid SF inside vesicle under sonication. SF-LIP is capable of encapsulating bFGF (SF-bFGF-LIP) with high efficiency, having a diameter of 99.8 +/- 0.5 nm and zeta potential of -9.41 +/- 0.10 mV. SF-LIP effectively improves the stability of bFGF in wound fluids. After 8 h of incubation with wound fluids at 37 degrees C, more than 50% of free bFGF are degraded, while only 18.6% of the encapsulated bFGF in SF-LIP are destroyed. Even after 3 d of preincubation with wound fluids, the cell proliferation activity and wound healing ability of SF-bFGF-LIP are still preserved but these are severely compromised for the conventional bFGF-liposome (bFGF-LIP). In vivo experiments reveal that SF-bFGF-LIP accelerates the wound closure of mice with deep second-degree scald. Moreover, due to the protective effect and enhanced penetration ability, SF-bFGF-LIP is very helpful to induce regeneration of vascular vessel in comparison with free bFGF or bFGF-LIP. The liposome with SF hydrogel core may be a potential carrier as growth factors for wound healing.
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