期刊
ACS SYNTHETIC BIOLOGY
卷 7, 期 2, 页码 392-398出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.7b00412
关键词
lignin degradation; biosensors; functional metagenomic screening
资金
- Natural Sciences and Engineering Research Council (NSERC) of Canada
- Canada Foundation for Innovation (CFI)
- Canadian Institute for Advanced Research (CIFAR)
- NSERC CREATE training program, Ecosystem Services, Commercialization Platforms and Entrepreneurship (ECO-SCOPE)
The discovery and utilization of biocatalysts that selectively valorize lignocellulose is critical to the profitability of next-generation biorefineries. Here, we report the development of a refactored, whole-cell, GFP-based biosensor for high-throughput identification of biocatalysts that transform lignin into specialty chemicals from environmental DNA of uncultivable archaea and bacteria. The biosensor comprises the transcriptional regulator and promoter of the emrRAB operon of E. coli, and the configuration of the biosensor was tuned with the aid of mathematical model. The biosensor sensitively and selectively detects vanillin and syringaldehyde, and responds linearly over a wide detection range. We employed the biosensor to screen 42 520 fosmid clones comprising environmental DNA isolated from two coal beds and successfully identified 147 clones that transform hardwood kraft lignin to vanillin and syringaldehyde.
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