期刊
ACS SYNTHETIC BIOLOGY
卷 6, 期 5, 页码 902-904出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.6b00343
关键词
CRISPR; Cas9; sgRNA activity prediction; sgRNA scorer; genome engineering
资金
- NIH [RM1 HG008525, P50 HG005550]
It has been possible to create tools to predict single guide RNA (sgRNA) activity in the CRISPR/Cas9 system derived from Streptococcus pyogenes due to the large amount of data that has been generated in sgRNA library screens. However, with the discovery of additional CRISPR systems from different bacteria, which show potent activity in eukaryotic cells, the approach of generating large data sets for each of these systems to predict their activity is not tractable. Here, we present a new guide RNA tool that can predict sgRNA activity across multiple CRISPR systems. In addition to predicting activity for Cas9 from S. pyogenes and Streptococcus thermophilus CRISPR1, we experimentally demonstrate that our algorithm can predict activity for Cas9 from Staphylococcus aureus and S. thermophilus CRISPR3. We also have made available a new version of our software, sgRNA Scorer 2.0, which will allow users to identify sgRNA sites for any PAM sequence of interest.
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