期刊
ACS SYNTHETIC BIOLOGY
卷 6, 期 10, 页码 1962-1968出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.6b00354
关键词
binary T-DNA vector; pBBR1; Agrobacterium tumefaciens; plant biotechnology; multigene transfer; ethanol-inducible switch; Potyviridae; Ugandan cassava brown streak virus
资金
- Spanish Ministerio de Ciencia y Economia [BIO2013-49053-R, BIO2013-42193-R, BIO2016-80572-R, Plant KBBE PCIN-2013-056]
Improved plants are necessary to meet human needs. Agrobacterium-mediated transformation is the most common method used, to rewire plant capabilities. For plant gene delivery, DNA constructs are assembled into binary T-DNA vectors, that rely on broad host range origins for bacterial replication. Here we present pLX vectors, a set of mini binary T-DNA plasmids suitable for Type ITS restriction endonuclease- and overlap-based assembly methods. pLX vectors include replicons from compatible broad host range plasmids. Simultaneous usage of pBBR1- and RK2-based pLX vectors in a two-plasmid/one Agrobacterium strain strategy allowed multigene delivery to plants. Adoption of pLX vectors will facilitate routine plant transformations and targeted mutagenesis, as well as complex part and circuit characterization.
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