期刊
ACS SYNTHETIC BIOLOGY
卷 6, 期 7, 页码 1359-1369出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.6b00397
关键词
transcriptional noise; bet hedging; Pseudomonas putida; intracellular heterogeneity; TOL plasmid
资金
- CAMBIOS Project of the Spanish Ministry of Economy and Competitiveness [RTC-2014-1777-3]
- HELIOS Project of the Spanish Ministry of Economy and Competitiveness [BIO 2015-66960-C3-2-R]
- European Union [ERC-2012-ADG-322797, EU-H2020-BIOTEC-2014-2015-6335536, 704410-H2020-MSCA-IF-15]
Gene expression noise is not only the mere consequence of stochasticity, but also a signal that reflects the upstream physical dynamics of the cognate molecular machinery. Soil bacteria facing recalcitrant pollutants exploit noise of catabolic promoters to deploy beneficial phenotypes such as metabolic bet-hedging and/or division of biochemical labor. Although the role of upstream promoter-regulator interplay in the origin of this noise is little understood, its specifications are probably ciphered in flow cytometry data patterns. We studied Pm promoter activity of the environmental bacterium Pseudomonas putida and its cognate regulator XyIS by following expression of Pm-gfp fusions in single cells. Using mathematical modeling and computational simulations, we determined the kinetic properties of the system and used them as a baseline code to interpret promoter activity in terms of upstream regulator dynamics. Transcriptional noise was predicted to depend on the intracellular physical distance between regulator source (where XylS is produced) and the target promoter. Experiments with engineered bacteria in which this distance is minimized or enlarged confirmed the predicted effects of source/target proximity on noise patterns. This approach allowed deconvolution of cytometry data into mechanistic information on gene expression flow. It also provided a basis for selecting programmable noise levels in synthetic regulatory circuits.
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