期刊
ACS SYNTHETIC BIOLOGY
卷 6, 期 8, 页码 1453-1460出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.7b00078
关键词
inteins; alpha-factor; peptide analogues; cell signaling; gene regulation
资金
- NSF [CBET1263719]
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [1263719] Funding Source: National Science Foundation
Synthetic control strategies using signaling peptides to regulate and coordinate cellular behaviors in multicellular organisms and synthetic consortia remain largely underdeveloped because of the complexities necessitated by heterologous peptide expression. Using recombinant proteins that exploit split intein-mediated reactions, we presented here a new strategy for reconstituting functional signaling peptides capable of eliciting desired cellular responses in S. cerevisiae. These designs can potentially be tailored to any signaling peptides to be reconstituted, as the split inteins are promiscuous and both the peptides and the reactions are amenable to changes by directed evolution and other protein engineering tools, thereby offering a general strategy to implement synthetic control strategies in a large variety of applications.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据