期刊
ACS SYNTHETIC BIOLOGY
卷 7, 期 1, 页码 240-248出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.7b00289
关键词
synthetic biology; optogenetics; near-infrared light; bacteriophytochrome
资金
- Office of Naval Research [MURI N000141310074]
- Agency for Science, Technology and Research (A*STAR), Singapore
Optogenetics is a technology wherein researchers combine light and genetically engineered photoreceptors to control biological processes with unrivaled precision. Near-infrared (NIR) wavelengths (>700 nm) are desirable optogenetic inputs due to their low phototoxicity and spectral isolation from most photoproteins. The bacteriophytochrome photoreceptor 1 (BphP1), found in several purple photosynthetic bacteria, senses NIR light and activates transcription of photosystem promoters by binding to and inhibiting the transcriptional repressor PpsR2. Here, we examine the response of a library of output promoters to increasing levels of Rhodopseudomonas palustris PpsR2 expression, and we identify that of Bradyrhizobium sp. BTAi1 crtE as the most strongly repressed in Escherichia coli. Next, we optimize Rps. palustris bphP1 and ppsR2 expression in a strain engineered to produce the required chromophore biliverdin IX alpha in order to demonstrate NIR-activated transcription. Unlike a previously engineered bacterial NIR photoreceptor, our system does not require production of a second messenger, and it exhibits rapid response dynamics. It is also the most red-shifted bacterial optogenetic tool yet reported by approximately SO nm. Accordingly, our BphP1-PpsR2 system has numerous applications in bacterial optogenetics.
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