期刊
BIOORGANIC & MEDICINAL CHEMISTRY
卷 25, 期 10, 页码 2730-2742出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmc.2017.03.041
关键词
SABRE; Hyperpolarization; MRI; Real-time imaging; L-Arginine
资金
- University of Aberdeen's Development Trust
- Wellcome Trust [92506, 098335]
Hyperpolarization enhances the intensity of the NMR signals of a molecule, whose in vivo metabolic fate can be monitored by MRI with higher sensitivity. SABRE is a hyperpolarization technique that could potentially be used to image nitric oxide (NO) production in vivo. This would be very important, because NO dysregulation is involved in several pathologies, including cardiovascular ones. The nitric oxide synthase (NOS) pathway leads to NO production via conversion of L-arginine into L-citrulline. NO is a free radical gas with a short half-life in vivo (approximate to 5 s), therefore direct NO quantification is challenging. An indirect method - based on quantifying conversion of an L-Arg - to L-Cit-derivative by H-1 NMR spectroscopy is herein proposed. A small library of pyridyl containing L-Arg derivatives was designed and synthesised. In vitro tests showed that compounds 4a-j and 11a-c were better or equivalent substrates for the eNOS enzyme (NO2- production = 19-46 uM) than native L-Arg (NO2- production = 25 mu M). Enzymatic conversion of L-Arg to L-Cit derivatives could be monitored by 1H NMR. The maximum hyperpolarization achieved by SABRE reached 870-fold NMR signal enhancement, which opens up exciting future perspectives of using these molecules as hyperpolarized MRI tracers in vivo. (C) 2017 The Authors. Published by Elsevier Ltd.
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