期刊
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
卷 621, 期 -, 页码 31-37出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.abb.2017.02.012
关键词
Packed red blood cells; N-acetylcysteine; Oxidative stress; Glutathione; Peroxiredoxin-2; Thiol homeostasis
资金
- Comision Sectorial de Investigacion Cientifica, UdelaR [CSIC 007-348]
- Programa de Desarrollo de las Ciencias Basicas, PEDECIBA, Uruguay
- Agencia Nacional de Investigacion e Innovacion, ANII, Uruguay
Storage inflicts a series of changes on red blood cells (RBC) that compromise the cell survival and functionality; largely these alterations (storage lesions) are due to oxidative modifications. The possibility of improving the quality of packed RBC stored for transfusion including N-acetylcysteine (NAC) in the preservation solution was explored. Relatively high concentrations of NAC (20-25 mM) were necessary to prevent the progressive leakage of hemoglobin, while lower concentrations (>= 2.5 mM) were enough to prevent the loss of reduced glutathione during the first 21 days of storage. Peroxiredoxin-2 was also affected during storage, with a progressive accumulation of disulfide-linked dimers and hetero-protein complexes in the cytosol and also in the membrane of stored RBC. Although the presence of NAC in the storage solution was unable to avoid the formation of thiol-mediated protein complexes, it partially restored the capacity of the cell to metabolize H2O2, indicating the potential use of NAC as an additive in the preservation solution to improve RBC performance after transfusion. (C) 2017 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据