期刊
BIOENERGY RESEARCH
卷 10, 期 2, 页码 488-498出版社
SPRINGER
DOI: 10.1007/s12155-017-9814-y
关键词
Basfia succiniciproducens; Lignocellulosic biomass; Arundo donax; Succinic acid; Fermentation
资金
- Ministero dell'Universita e della Ricerca Scientifica [PON03PE_00107_1/1, 713/Ric]
Bio-based succinic acid production from lignocellulosic biomass is one of the attractive and prominent alternative technologies to overcome issues associated with the utilization of fossil sources. In this context, it is necessary to find new microorganisms that are able to efficiently ferment this recalcitrant feedstock. The ecological approach developed in this study enabled the isolation of Basfia succiniciproducens BPP7 from a complex rumen ecosystem. This new wild-type strain was able to synthesize up to 6.06 +/- 0.05 g/L of succinate (corresponding to 0.84 +/- 0.017 g of succinate per gram of consumed glucose + xylose and to 0.14 +/- 0.001 g of succinate per gram of glucans + xylans present in the biomass before hydrolysis) from Arundo donax hydrolysate in separate hydrolysis and fermentation (SHF) experiments. Higher titers of succinic acid were obtained through the optimization of growth conditions. The optimal medium composition identified on the smaller scale was then used for 2.5-L batch experiments, which used A. donax hydrolysate and yeast extract as the main C and N sources, respectively. A maximal titer of 9.4 +/- 0.4 g/L of succinic acid was obtained after 24 h. The overall results clearly demonstrate the potential of B. succiniciproducens BPP7 for succinate production.
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