期刊
ANALYTICAL METHODS
卷 9, 期 17, 页码 2511-2516出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7ay00544j
关键词
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资金
- National Natural Science Foundation of China [11672095, 11372093]
- Self-Planned Task of the State Key Laboratory of Robotics and System (HIT) [201510B, SKLRS201606C]
- Foundation for Innovative Research Groups of the National Natural Science Foundation of China [51521003]
This work reports a simple microfluidic method for one-step encapsulation of two reagents with varying concentrations in water-in-oil-in-water (W/O/W) double-emulsion drops. This method not only enables nanoliter-scalere actions and analyses under a series of controlled concentrations of two reagents without stopping the experiments or changing solutions, but also protects the reactions from external disturbance for an extended amount of time by the core-shell structure. To achieve this, a capillary device embedded with a theta-shaped tube is fabricated to produce monodisperse emulsion drops, in which the concentrations of the reagents encapsulated are varied by tuning the flow rates in the two individual channels of the theta tube. The relative volume ratio of the encapsulated reagents can reach up to 1 : 20. In addition, microcapsules converted from emulsion drops have excellent long-term robustness. As a proof of concept, we conduct two frequently used reactions at the nanoliter scale with varying concentrations: acid-base reaction and enzyme-catalyzed redox reaction for glucose detection.
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